Description
Description: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of SA in Guinea pig serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Guinea pig SA. During the reaction, Guinea pig SA in the sample or standard competes with a fixed amount of Guinea pig SA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Guinea pig SA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Guinea pig SA in the samples is then determined by comparing the O.D. of the samples to the standard curve